Inositol pyrophosphates have been linked to a wide range of cellular functions. Many of them are synthesized by inositol hexakisphosphate kinase (IP6K) from two natural substrates, inositol pentakisphosphate (IP5) and inositol hexakisphosphate (IP6). We structurally analyzed IPs synthesized by three mammalian isoforms of IP6K from IP5 and IP6 thereby revealing a number of products with specific stereochemistry of added phospho-anhydride bonds. Also, IP6K synthesizes pyrophosphate as well as triphospho groups on the inositol ring. IP6K shares the same activities both in vitro and in vivo.
COBISS.SI-ID: 3889434
At this meeting we (U. Dermol) presented our work on cyclic nucleotide phosphodiesterases. Since the meeting was structurally oriented, being focused on X-ray crystallography as methodology, we showed the part of our work connected to protein sample preparation, crystallization, X-ray diffraction data collection and determination of crystal structures. We also discussed results on both bacterial and eukaryotic proteins from the same class of phosphodiesterases obtained in our group.
COBISS.SI-ID: 4105754