The study was aimed at assessing the ability of CpG ODNs class C applied as a single agent and in combination with radiotherapy to induce the anti-tumor immunity in experimental tumor model in mice (subcutaneous – s.c. B16F1). The combined therapy (CpG ODNs and tumor irradiation) remarkably enhanced the anti-tumor effect. The peritumoral (p.t.) application of CpG ODNs in combination with irradiation increased the number of dendritic cells (DCs) at the tumor site and improved the antigen loading and maturation of DCs.
COBISS.SI-ID: 819835
The aim of this study was to isolate putative stem cells from OSE scrapings, to set-up an OSE cell culture, to follow the in vitro oogenesis and possible formation of parthenogenetic embryos in 21 postmenopausal women with no naturally present follicles and oocytes. Cells were separated by density gradient centrifugation and grown in vitro, where they proliferated and formed embryoid body-like structures. Their markers of pluripotency such as telomerase activity were decreased during in vitro culture and they did not form teratoma after the injection into SCID mice.
COBISS.SI-ID: 714875
This study was aimed at assessing the ability of tumor vaccine composed of C-class CpG ODNs and irradiated melanoma tumor cells B16F1 followed by two additional injections of CpG ODNs to induce the generation of a functional long-term memory response in experimental tumor model in mice (i.p. B16F1). We demonstrated that tumor vaccine followed by two additional injections of CpG ODNs induces a long-term immunity (duration at least 8 months) against aggressive B16F1 tumors.
COBISS.SI-ID: 995195
In this study, 239 samples obtained from 215 patients with metastatic colorectal cancer were tested for the presence of the seven most common mutations in the KRAS gene and the V600E mutation in the BRAF gene. Among the tested patients 46.2% were KRAS mutants. Around five percent (5.1%) of the tested patients bore the V600E mutation in BRAF gene. All the patients showing to have the V600E mutation in BRAF were wt-KRAS.
COBISS.SI-ID: 982395
Study was aimed to investigate (1) maturation of APCs at the molecular level by following the expression of CD11c, CD86 and MyD88 genes, (2) generation of memory T cells, and (3) the tissue distribution of the (mature) APCs and memory cells after treatment of mice with a tumor vaccine composed of C-class CpG ODNs and irradiated melanoma B16F1 tumor cells. Our results provide insight into the nature and scope of the antitumor immune response elicited by this kind of tumor vaccine in vivo.
COBISS.SI-ID: 1077371