We investigated whether the level of fused late endocytic compartments, which are part of antigen presenting pathway affects the immunostimulatory capacity of DC-TC hybridomas. Samples of electrofused and control cells were co-cultured with cytotoxic T cells. The results showed that cytotoxic T cell responses are enhanced, if a higher percentage of fused late endocytic compartments is present in the cell population of electrofused samples. We concluded that the level of fused lysosomes in electrofused samples significantly affect the immunostimulatory capacity of hybridomas.
COBISS.SI-ID: 25624281
Pathophysiologic implications of intermediate filament (IF) proteins up-regulation in astrocytes remain incompletely understood. We studied whether the trafficking of recycling vesicles and endosomes/lysosomes depends on IFs. The findings show that astrocyte IFs differentially affect mobility of vesicles in WT astrocytes and in glial fibrillary acidic protein and vimentin deficient astrocytes; the most prominent changes were observed in endosomes/lysosomes. We propose that up-regulation of IFs in pathologic states may alter the function of astrocytes by deregulating vesicle trafficking.