A solution state NMR study has shown that d(G4T3G4) in the presence of 15NH4+ ions folds into a single bimolecular G-quadruplex structure in which its G-tracts are antiparallel and the two T3 loops span along the edges of the outer G-quartets on the opposite sides of the G-quadruplex core. Molecular models based on NMR data demonstrate that thymine loop residues efficiently base-base stack on the outer G-quartets. The use of heteronuclear NMR enabled us to localize three 15NH4+ ion binding sites between pairs of adjacent G-quartets and study the kinetics of their movement.
COBISS.SI-ID: 4051738
We have determined a NMR solution structure of a model mimicking the interaction of let-7 miRNA with its complementary site (LCS 2) in the 3’ untranslated region (3’-UTR) of the lin-41 mRNA. A structural study was performed by NMR spectroscopy using NOE restraints, torsion angle restraints and residual dipolar couplings. The 33-nt RNA construct folds into a stem-loop structure that features two stem regions which are separated by an asymmetric internal loop.
COBISS.SI-ID: 3875610