Drosophila gene knockout approach has revealed the role of Rab35 protein in the actin assembly. Using proteomic analysis we have identified fascin as the key ligand which interacts with Rab35 and mediates actin bundling. Through the interaction with fascin, Rab35 regulates the actin assembly during bristle development in Drosophila and filopodia formation in cultured cells. Proteomic identification of interaction between fascin and Rab35 was performed using afinity chromatobrapy with immobilized recombinant Rab35. This approach will also be applied to this project.
COBISS.SI-ID: 22849063
Mouse is the most commonly used animal model for human disease study. However, results from mouse models cannot always be directly related to human disease. In this research we have shown that human and murine cathepsin B exhibited similar biochemical properties, indicating that cathepsin B transgenic mouse models could be useful for studying its role in human pathologies.
COBISS.SI-ID: 22472999