We evaluated genotoxicity/cytotoxicity of native samples of wastewaters (15), surface waters (28) and potable waters (8) with the bacterial SOS/umuC assay and MTT and comet assay with human hepatoma HepG2 cells. The results showed that the application of the SOS/umuC assay and MTT assay with HepG2 cells was suitably sensitive combination of assays to monitor genotoxicity/cytotoxicity of native samples of wastewaters and surface waters, and that bioassays should be an integral tool in the evaluation of toxicity of complex waste and surface water samples.
COBISS.SI-ID: 22520359
In order to optimise the extraction method for simultaneous determination of butyl-, phenyl- and octyltin compounds in sewage sludge five different extractants (tetramethylammonium hydroxide, HCl in methanol, glacial acetic acid, mixture of acetic acid and methanol (3:1), and mixture of acetic acid, methanol and water (1:1:1), the presence or not of a complexing agent (tropolone), and the use of different modes of extraction (mechanical stirring, microwave and ultrasonic assisted extraction) were tested.
COBISS.SI-ID: 23116327
A rapid analytical method for the simultaneous determination of 12 different organotin compounds (OTC): methyl-, butyl-, phenyl- and octyl-tins in natural water samples was developed. It comprises of in situ derivatisation (by using NaBEt4) of OTC in salty or fresh water sample matrix adjusted to pH 6 with Tris-citrate buffer, extraction of ethylated OTC into hexane, separation of OTC in organic phase on 15 m GC column and subsequent quantitative determination of separated OTC by ICP-MS.
COBISS.SI-ID: 24539175
In published paper we developed T-RFLP analyses of 16SrRNA genes. In addition we have also prepared 16S rRNA gene libraries from DNA isolated from the untreated biofilm. Described molecular biological study was performed on selected pharmaceuticals, which were applied as model compounds. Research results were applied to selected endocrine disrupter compounds (E1, E2, EE2 in E3), which are subject of this project.
COBISS.SI-ID: 3484024
A comprehensive study of the parameters influencing the detection of organotin compounds (OTCs) by a pulsed flame photometric detector (PFPD) in sewage sludge was performed. Operating conditions of a PFPD system was optimised to eliminate spectral interferences.
COBISS.SI-ID: 21585703