Platelets contain over 14 different growth factors implied in the SC development and differentiation. Authors extensively describe all aspects of their biological functions, their praparation and their clinical implications.
COBISS.SI-ID: 23866329
Ovarian stem cells (OSCs) were isolated from the OSE layer in women. Their diameters is 2-4 um. They expressed early embryonic developmental markers SSEA4, Oct-4, Nanog, Sox-2, and c-kit. When grown in vitro, oocyte-like cells developed, with diameter 95 um and expressed Oct-4A, Oct-4B, c-kit, VASA, and ZP2 transcription markers. A new term "embryonic-like stem cells of the adult" (ESC-A) was proposed for these cells that might persist in various tissues and organs of adults. These findings could be used for autologous treatment of ovarian infertility and degenerative diseases.
COBISS.SI-ID: 24354265
Human hMSCs from bone marrow were cultured. Cartilage and bone formation were assessed by magnetic resonance imaging and micro-computerized tomography imaging and by biochemical, histological and mechanical assays. Bone-like tissue formation progressed more than cartilage-like one, suggesting that tissue composites with well-mineralized regions can be generated in vitro, that hMSCs have markedly higher capacity for producing engineered bone than cartilage, and that chondrogenic factors play major roles at early stages of bone formation by hMSCs.
COBISS.SI-ID: 24142041
We constructed half-centimeter-sized bone constructs in vitro by using human adipose-derived stem cells (hASCs) from adult donors. hASCs were seeded on native bone scaffolds, providing the environment for osteogenic differentiation, and cultured in perfusion bioreactors for 5 weeks. Addition of osteogenic factors significantly increased the cellularity and the amounts of bone matrix components (collagen, bone sialoprotein, and bone osteopontin), resulting in the formation of compact and viable bone tissue constructs that can be used for autologous transplantation.
COBISS.SI-ID: 25944537