We have focused at the small embryonic like stem cells in adult numan bone marrow and proved their existence. They were shown to express embryonic stem cell markers. Further she diferentiated the cells in the presence of growth factors into large oocyte like cells and with the gene expression analysis she has shown that oogenesis-specific genes are expressed. The results have confirmed the existence of stem cells with embryonic character in the adult human bone marrow.
D.10 Educational activities
COBISS.SI-ID: 56956093-D environment influences the growth of SCs. We compared the ESC and ASC growth on different 3-D hidrogels by means of live/dead assay, metabolic activity and gene expression analysis. Human ASCs grew optimally in collagen. Hidrogels offer new possibilities for SC expansion.
D.10 Educational activities
COBISS.SI-ID: 6036601The cultivation of ESCs is hindered by their differentiation. If they are grown on feeder layer composed of ASCs, they retail their embryonic character as proven by expression analysis of Oct-4, Sox-2 and Nanog.
D.10 Educational activities
COBISS.SI-ID: 6037625There is very little evidence that ESC could be normally found in the adult blood. With the expression analysis of pluripotent markers Oct-4 and Nanog we proved their feeble presence, indicating their eventual release from the bone marrow into the blood.
D.10 Educational activities
COBISS.SI-ID: 6037881In the UCB there are some ESC-like cells expressing the typical markers Oct-4, Nanog and SSEA-4. Me managed to analyse them by flow cytometry and to increase their numbers by immunomagnetic sorting. With the cell sorter we finally isolated a population expressing CD133, but lacking CD45 and other markers of lineage differentiation. Additional tests need to be performed in order to explore the possible use of these cells in regenerative medicine.
D.10 Educational activities
COBISS.SI-ID: 6421113