We compared two PCR methods and B. burgdorferi s.l. culture results from CSF and blood of patients with Lyme neuroborreliosis (LNB), suspected LNB, TBE and neurosurgical patients. Borrelial DNA was detected with at least one of the PCR methods in 11.9% of blood samples and 15.4% of CSF samples. The majority of strains were B. afzelii. Borreliae were isolated from 0.7% of blood samples and 3.2% of CSF samples. B. garinii was identified in all CSF isolates. There is a need for standardization of the methods for direct detection of borrelial infection. Appropriate control group has to be chosen.
F.22 Improvement to existing health/diagnostic methods/procedures
COBISS.SI-ID: 24856281We compared three different serological tests for detection of borrelial immune response: IFA, ELISA and LIAISON. For detection of borrelial IgM antibodies ELISA was found to be more sensitive than the other two tests, while LIAISON® was found to be more sensitive test for detection of IgG antibodies. Discrepancies in findings obtained on identical sera strongly indicate the need for standardisation of the serological methods for detection of Borrelia burgdorferi sensu lato infection.
F.22 Improvement to existing health/diagnostic methods/procedures
COBISS.SI-ID: 24355289