The creation of localized sites of increased molecular transport termed local transport regions (LTRs) can be observed during electroporation, as well as changes in the bulk electric properties of skin layers. We modeled these phenomena with a numerical model and compared the output of the model with our own in vivo experiments and previously published results of skin electroporation and a good agreement was obtained.
COBISS.SI-ID: 6572116
The electropermeabilization process in skin was described theoretically, by means of numerical modeling, leaning on data derived from our in vivo experiments previously published. The numerical models took into account the layered structure of skin, macroscopical changes of its bulk electric properties during electroporation, as well as the presence of localized sites of increased molecular transport termed local transport regions (LTRs).
COBISS.SI-ID: 6615124
As a complementary work to in vitro, in vivo and medical experiments, we can use analytical and numerical models to represent real biological phenomena of, in our case, electroporation. In this way we can evaluate different electrical parameters in advance, such as pulse amplitude, duration, number of pulses, or different electrode geometries. Such numerical models can contribute significantly to the understanding of an experiment and treatment planning as well as to the design of new electroporation devices and electrodes.
COBISS.SI-ID: 6697300
Gene electrotransfer is gaining momentum as an efficient methodology for nonviral gene transfer. Data suggest that electric pulses play two roles: permeabilizing the cell membrane and electrophoretically supporting the migration of DNA toward or across the permeabilized membrane. We investigated combinations of permeabilizing short high-voltage pulses (HV; hundreds of V/cm) and mainly electrophoretic long low-voltage pulses (LV; tens of V/cm) in muscle, liver, tumor, and skin in rodent models.
COBISS.SI-ID: 698235
We performed a series of in vivo experiments, delivering plasmids to rat skin with electroporation using external plate electrodes. The experiments showed that skin layers below stratum corneum can be permeabilized in this way. In order to study the course of skin tissue permeabilization by means of electric pulses, a numerical model using the finite element method was made. The model is based on the tissue-electrode geometry and electric pulses used in our in vivo experiments. The results obtained with the model are in good agreement with the in vivo results of gene transfection in rat skin.
COBISS.SI-ID: 6273876