The numerical models took into account the layered structure of skin, macroscopical changes of its bulk electric properties during electroporation, as well as the creation of localized sites of increased molecular transport termed local transport regions (LTRs). The output of the models was compared with the current and the voltage measured during in vivo experiments and a good agreement was obtained. Also, the voltage amplitudes suggested by the model are also well in the range of the voltage amplitudes reported by other authors to cause skin permeabilization, as well as our own experiments.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 6099284In order to study the course of skin tissue permeabilization by means of electric pulses, a numerical model was built, with COMSOL Multiphysics, using the finite element method. The model is based on in vivo experiments performed previously. We took into account the layered structure of skin and changes of its bulk electric properties during electroporation, as observed in the in vivo experiments. The results obtained with the model were then compared to the in vivo results of gene transfection in rat skin and a good agreement was obtained.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 5981268Passive electric properties of biological tissues are important in applied problems of electroporation. The current densities and pathways resulting from an applied electrical pulse are dictated to a large extent by the relative permittivity and conductivity of biological tissues. We briefly present some theoretical basis for the current conduction in biologic materials and factors affecting the measurement of tissue dielectric properties that need to be taken into account when designing the measurement procedure, as well as their changes during electroporation.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 6248532Skin electroporation was described theoretically, by means of numerical modeling, leaning on data derived from the in vivo experiments published previously, and tissue conductivity data found in literature. The results obtained with the model were compared to the in vivo results of gene transfection in rat skin and a good agreement was found. Further, tissue conductivities and model geometry were varied to estimate their effect on the output of the model. The changes in the output electric current were still well in the range of the currents measured during the vivo experiments.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 6569812We describe the usefulness of numerical modeling in biomedical applications of cell and tissue electroporation. Various parameters (current, voltage amplitude, field strength and orientation, electrode geometries...) can be evaluated, allowing us to design electrode geometries and electroporation protocols as a part of treatment planning. A good model in agreement with experimental results can offer useful insight into the understanding of biological processes. Also, they are sometimes the only possible or ethically acceptable alternative to experimenting on real biological systems.
B.04 Guest lecture
COBISS.SI-ID: 6483796