We presented a method for construction of numerical models of clusters of cells and the results of measurements of the induced transmembrane voltage and electroporation, which were performed on the same cells from which the model was built. Comparison of measurements and calculations showed that cells in clusters respond differently to the electric field exposure under various field parameters (either as one giant interconnected cell or as individuall cells), which is probably a consequence of opening and closing of intracellular connections.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 5976404The award is presented to a young doctorand of the Faculty of Electrical Engineering for his outstanding research work in his doctoral thesis, shown by the published papers and citations of his work.
E.01 National awards
In the frame of the International scientific workshop and postgraduate course "Electroporation based technologies and treatments" we demonstrated the method for monitoring the course of electroporation of cell membranes. The method uses the fluorescent Ca indicator Fura-2AM for monitoring the uptake of Ca ions into the cell, which occurs due to electroporation. In the practical example we demonstrated how the method can be used to determine the influence of cell shape and cell orientation on the efficiency of electroporation.
F.18 Transfer of new know-how to direct users (seminars, fora, conferences)
COBISS.SI-ID: 6251348We presented a method for upgrading the existing steady-state numerical model of the induced transmembrane voltage (ITV) and electroporation, which allows the calculation of the time-dependent changes of the ITV and electroporation. A variable membrane conductivity was introduced into the model, which accounts for the changes of the electrical properties of the membrane due to electroporation.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 6569556We examined the influence of gap junctions on the induced transmembrane voltage and electroporation of cells in clusters. When exposed to long, low voltage pulses nonihibited cells in clusters behaved as one giant, interconnected cell. At short, high voltage pulses they behaved as individual cells. Different response of cells was attributed to the opening and closing of gap junctions. This was demonstrated by pre-treating the cells with gap junction inhibitor Oleamide, which caused the cells in clusters to always respond as individual cells, regardless of the pulse parameters.
B.03 Paper at an international scientific conference