In the published study we explored the effect of MCLR on the expression of selected genes involved in the cell response to DNA damage and apoptosis. The HepG2 cells were exposed to non-toxic MCLR concentrations and the mRNA expression was determined with the qrt-PCR. MCLR elevated the expression of tumour suppressor gene p53 and its downstream regulated genes involved in DNA repair and cell cycle regulation (p21, gadd 45a, mdm2), as well as the pro-apoptotic gene bax, while it decreased anti-apoptotic bcl-2. These results support the previous suggestion that MCLR is a genotoxic carcinogen.
COBISS.SI-ID: 1820239
In the published study we investigated the effect of non cytotoxic concentrations of MCLR on generation of reactive oxygen species (ROS) and DNA damage in human colon adenocarcinoma CaCo-2, human astrocytoma IPDDC-A2 and human B lymphoblastoid NCNC cell lines. The viability of CaCo-2 cells was reduced, while the viability of NCNC and IPDDC-2A cells was not affected. MCLR at non cytotoxic concentrations induced time and dose dependent increase of DNA damage only in CaCo-2 cells. These results indicate that apart to liver also colon should be considered as the target of microcystin toxicity.
COBISS.SI-ID: 1889871