HPLC methods with VIS and MS detection for determination of biologically active shikonin derivatives were developed and enabled for the first time simultaneous separation of all nine shikonin derivatives (including positional and geometric isomers), which were identified in the root epidermis of plant Echium italicum L. (Boraginaceae) for the first time.
COBISS.SI-ID: 4134682
The basic structural and chromatographic properties of short monolithic analytical columns were presented for the first time.
COBISS.SI-ID: 3469176