We developed new HPLC-UV, LC-MS and TLC methods for identification and determination of triterpenoids and phytosterols in samples of plant origin. We studied chromatographic separation of ten triterpenoids (many of them structural isomers) and two phytosterols. We isolated commercially not available triterpenoid ?-amyrin from tomato fruit. The combination of three TLC methods (using silica gel and C18 RP layers) and two HPLC methods (C18 RP) enables qualitative determination of all 12 compounds and proves to be useful for the analysis of plant extracts.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 4011290An HPLC method developed for determination of all-trans-lutein in food supplements was used for the analysis lutein in 15 different food supplements (tablets and capsules). The results proved the necessity of the determination of lutein in food supplements: 4 samples contained no lutein at all, 3 samples contained about 140 % and only 6 samples were in the 85-115% range of the declared content, 2 samples contained about 51% of the declared value of lutein in the form of lutein esters. Some cis isomers of lutein and small amounts of all-trans-zeaxanthin were also detected in all samples.
F.02 Acquisition of new scientific knowledge
COBISS.SI-ID: 4030234