The paper describes glucosamine-6-phosphate deaminase characteristics which competes with 6-phosphofructo-1-kinase for identical substrate fructose-6-phosphate in Aspergillus niger cells. We have shown that deaminase from A. niger possesses specific kinetic features in respect to the enzymes form other organisms, enabling fast formation of glucosamine. Enzyme activity therefore decreases metabolic flux through glycolysis at the early stages of A. niger growth.
COBISS.SI-ID: 3885082
At the 13th International Biotechnology Symposium and Exibition, that is held each four years, the use of the gene for synthesising the shorter fragment of 6-phosphofructo-1-kinase has been presented. The gene, while expressed in specific recipient organism causes the deregulation of metabolic flux through glycolysis which results in enhanced overall anabolic reactions in the cells. The gene might increase productivity and yields of various bio-products after insertion into commercial microorganisms.
COBISS.SI-ID: 4039962