Astrocites utilize regulated release of various signaling molecules stored in the vesicular lumen. Here were described the properties of exocytotic release of three classes of gliotransmitters: aminoacids, nucleotides and peptides.
We have monitored the yield of hybridoma cell formation by measuring the fusion of lysosomes, organelles involved in antigen presentation. The results demonstrate that lysosomal fusion occurs in hybridomas, which is time- and temperature-dependent. This approach therefore provides a new method for the hybridoma cell vaccine evaluation, which is based on the measurement of the level of lysosomal fusion, the intracellular physiological mechanism of antigen presentation.
COBISS.SI-ID: 23815129
We investigated whether the level of fused late endocytic compartments, which are part of antigen presenting pathway affects the immunostimulatory capacity of DC-TC hybridomas. Samples of electrofused and control cells were co-cultured with cytotoxic T cells. The results showed that cytotoxic T cell responses are enhanced, if a higher percentage of fused late endocytic compartments is present in the cell population of electrofused samples. We concluded that the level of fused lysosomes in electrofused samples significantly affect the immunostimulatory capacity of hybridomas.
COBISS.SI-ID: 25624281
In this article we review and comment latest approaches and techniques used for preparing large amounts of antigen-specific CTLs, suitable for clinical use.