Proteases, in particular, are known for their multilayered post-translational activity regulation that can lead to a significant difference between protease abundance levels and their enzyme activity. In this article present structural features of activity-based probes for proteases and discuss their applications in proteomic profiling of various catalytic classes of proteases.
COBISS.SI-ID: 22136615
We have used FRET (fluorescence resonance energy transfer) method to determine whether stefin B interacts with truncated form M75 of cathepsin L in living cells. Previously, it has been shown that both proteins localise to the nucleus. We have shown for the first time that stefin B interacts with cathepsin L in the living cells.
COBISS.SI-ID: 22186535