We designed a material with a surface-bound antimicrobial peptide for potential use in medical devices such as implants or catheters to prevent bacterial biofilm formation. A model substrate was coated by a layer of alkoxysilane GPTMS as a linker for coupling the peptide PMB with antibiotic activity against Gram-negative bacteria. After incubation of the coated glass in the solution with PMB two additional peaks, characteristic for the peptide, were observed in the IR spectra. By the biomaterial concentration of E. coli was reduced for up to 100.000 CFU/ml per cm2 of the material’s surface.
F.02 Acquisition of new scientific knowledge
COBISS.SI-ID: 3951386We designed and analyzed effective antimicrobial peptides and peptidomimetics with a broad spectrum of activity on bacteria, ability of neutralization of endotoxin and low toxicity for humans. The lead compound was LF11, a short peptide fragment from the N-terminus of human lactoferrin. Peptide ability to bind polysaccharide was confirmed in vitro and in cells. Using antimicrobial peptides the level of inflammatory cytokines was successfully decreased. We prepared a system for production of biologically active recombinant peptide via the formation of an insoluble fusion protein.
D.09 Tutoring for postgraduate students
COBISS.SI-ID: 2407281