High-voltage, nanosecond electric pulses can permeabilize membranes of cell organelles. In the paper, we described the electropermeabilization of endocytotic vesicles in B16 F1 mouse melanoma cells, observed by a fluorescence mycroscopy. With the use of two fluorescent dyes (lucifer yellow and propidium iodide) we found that the permeabilization of internal cell membranes by pulses of chosen parameters (1-20 pulses, 60 ns, 50 kV/cm, 1 kHz) is always accompanied by a permeabilization of plasma membrane. The method can be useful for introducing various substances into the cell.
COBISS.SI-ID: 7642708
Blumlein generators are used for various purposes: in radars, lasers, and recently, also in medical research for generation high-voltage, nanosecond electric pulses which can be used for electroporation of internal membranes of cells. In this paper, we described the structure and function of Blumlein generator that can achieve a high-repetition rate (up to 100 kHz) and variable duration and polarity of pulses. We also described the effects of these pulses to cells – the poration of endocytotic vesicles and plasma membrane.
COBISS.SI-ID: 7346772
High-voltage, nanosecond electric pulses (up to few hundreds nanoseconds, electric field strength of a few tens of kV/cm) can induce voltage at internal membranes of cells. This leads to permeabilization of organelle membranes. Effects of these pulses at internal membranes can exceed to those at plasma membrane. In the review article, we described various effects of nanosecond pulses on cells (permeabilization of vesicle membranes, release of calcium ions, release of cytochrome c from mitochondria, DNA damage, apoptosis) and their important role in biomedicine and biotechnology was discussed.
COBISS.SI-ID: 25426905