We clearly defined, by the use of uroplakins, parts of urothelium lined by urothelium. We have defined also junctional zones between urothelium and other epithelia of urinary tract. Urothelial cells do not reach terminal differentiation in every zones. This heterogeneity is possibly the basis for alternative courses of certain urothelial diseases.
COBISS.SI-ID: 19282649
We have shown that urothelium is divided into three cell lineages. During vitamin A deficiency, keratinized epithelium from proximal urethra expands into urinary bladder. This article explains the cellular basis for urothelial squamous metaplasia. It also suggests that human ureteral urothelial cells may be used as a convenient substitute for bladder urothelium in bladder reconstruction and augmentation.
COBISS.SI-ID: 20708569
Ultrastructural and immunocytochemical studies have revealed that restored tight junctions are the earliest cellular event during the urothelial superficial and full-thickness wound-healing process. The original terminally differentiated superficial urothelial cells contribute to the urothelium wound healing by developing tight junctions with de novo differentiated superficial cells and by stretching, thus providing a large urothelial surface with asymmetric unit membrane plaques.
COBISS.SI-ID: 19284697
Cells located at the forefront of a wound margin, play a significant role in coordinating the wound healing process. Early expression of ZO-1, claudin-4 and occludin was detected there. This expressions suggests that those cells may develop into fully differentiated superficial cells and finally restore blood-urine permeability barrier.
COBISS.SI-ID: 21167577
Regenerating urothelium after destruction with cyclophosphamide was used to simulate de-novo differentiation of cells. Cytokeratin 7 and uroplakins co-localized in the subapical cytoplasm of superficial cells from the early stage of differentiation on. Cytokeratin 7 become organized into a continuous network later, while cytokeratin 20 incorporating into the pre-existing trajectorial cytokeratin at the end of the differentiation. The differentiation of urothelial cells starts with the synthesis of differentiation-related proteins and later with their specific organization.
COBISS.SI-ID: 17565657