The experimental results together with a theoretical explanation for the basis for efficiency improvement of the Cliniporator(TM) device developed in cooperation with our EU partners. The device uses a custom-designed algorithm to detect the change of suspension/tissue impedance due to increased membrane permeability and adjust the amplitude of the electric pulses delivered. The articles received 12 pure citations so far, and an international patent was issued for the device and the algorithm.
COBISS.SI-ID: 4785492
Increase of the pulse repetition frequency reduces the unpleasant sensations during the pulse delivery in electrochemotherapy, and additionally increases the accuracy of electrode positioning due to reduced number of muscle contractions. It has already been implemented in the Cliniporator(TM) device. The articles received 7 pure citations so far, and an international patent was issued for the device that reduces the muscle contractions and unpleasant sensations by the use of high pulse repetition frequencies.
COBISS.SI-ID: 4659796
The results published in these three journal articles are the first to account for the changes of tissue conductivity caused by electroporation of the tissue. For this we had to write custom libraries and algorithms for the existing computational software. The sequential determination of the electroporated areas within the tissue is considerably more accurate than a single static/steady state evaluation. The articles received 18 pure citations so far.
COBISS.SI-ID: 4799572
Several recent experimental studies reported selective poration of organelle membranes with extremely short (tens to hundreds of ns) and strong (MV/m) electric pulses. We showed theoretically that such pulses can indeed induce voltages on the organelle membranes that temporarily exceed their counterpart on the plasma membrane, but only under very specific conditions (higher conductivity of organelle interior in comparison to the cytosol, lower permittivity of the organelle membrane in comparison to the plasma membrane) which are questionable. The article received 13 pure citations so far.
COBISS.SI-ID: 5106772
We describe a method for building realistic 3D finite-elements models of irregularly shaped biological cells. The cross-sections of the cell are photographed and joined into a 3D object. The membrane, which due to its thinness represents an extreme difficulty in finite-elements modeling, is replaced by a boundary condition of surface conductivity. The method and the models built were validated by comparing the computational results to the analytic predictions in spherical cells and potentiometric measurements in various irregularly shaped cells. The article received 12 pure citations so far.
COBISS.SI-ID: 5244244