Magnetic nanoparticles exhibit extraordinary properties, which make them excellent candidates for biomedical applications. In the present work, we report the engineering of novel temperature-sensitive magneto-liposomes for photothermally-induced drug release and MR imaging. Structural characterization using TEM and DSC revealed that small (4.5nm) nitrodopamine palmitate (NDPM)-coated magnetic nanoparticles (NPs) were successfully incorporated into the bilayer of lysolipid containing magneto liposomes without compromising DOX loading. More interestingly, our data successfully showed that low-energy NIR laser induced hyperthermia at much lower magnetic NPs concentrations (ug/ml), compared to magnetic hyperthermia ()10mg/ml). In support of this, DOX release from our magneto-liposomes was triggered using low-energy NIR laser (800 nm), resulting in quick and efficient on-demand drug release ()90% in 5 minutes). Furthermore, our lysolipid-containing magnetoliposomes revealed as a highly efficient T2 MRI contrast agent with 300-higher relaxivity ratio r2/r1 compared to free hydrophilic iron oxide NPs. In conclusion, we report the development of our novel magnetoliposomes that could be used in combination with NIR laser, offering efficient hyperthermia treatment, and on-demand drug release, besides being a highly sensitive T2 MRI imaging agent for image-guided drug delivery.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 31759143In the invited lecture, I presented the factors that influence nanoparticle relaxation and the strategy for optimizing them. Up to now, only few iron oxide nanoparticles (IO NPs) were clinically approved for MRI imaging. However, the majority of them have been withdrawn from the market either due to safety concerns or lack of profits. Therefore, there is a need for novel IO NPs-based imaging agents with high safety margin and superior MRI properties. Here we propose use of liposomal formulation for the preparation of efficient IO-based T2 contrast agents. In this talk, a systematic study on the influence of different phospholipids on the relaxivity r2 values of magneto-liposomes containing magnetic NPs in the bilayer will be shown and the explanation for the correlation between the bilayer fluidity, the NPs encapsulation efficiency (EE) and relaxivity r2 will be given. The efficacy of as-developed liposomes will be demonstrated with in vitro experiments as well.
B.04 Guest lecture
COBISS.SI-ID: 32168743