High intensity pulsed electromagnetic fields (HI-PEMF) can be used for contactless permeabilization of biological cells and thus, exploited for drug and gene delivery or other biomedical applications. Nevertheless, the availability of applicable technological setups is almost non-existent. In this work, we present a new prototype of HI-PEMF generator with generator circuit, parameters, characteristics and recommendations for future HI-PEMF systems.
COBISS.SI-ID: 12919380
An emerging noninvasive, contactless alternative to conventional electroporation are High Intensity Pulsed Electromagnetic Fields (HI-PEMF), whereby the electric field inside the tissue is induced remotely by external pulsed magnetic field. However, one of the current major drawbacks of HI-PEMFs is their inferior efficiency compared to conventional electroporation. In this study we present the proof-of-concept that by adding highly conductive 5 and 20 nm gold nanoparticles, we can significantly potentiate the permeabilizing effect of HI-PEMFs, making it possible to permeabilize up to 80% of the cells with minimal or no effect on cell survival, compared to negligible percentage of permeabilized cells using HI-PEMF alone.
COBISS.SI-ID: 32990759
The review provides an in-depth overview of the state-of-the-art knowledge of the biophysical mechanisms that participate in the increased membrane permeability triggered by the electric field.
COBISS.SI-ID: 12365652