Cell transplantation traditionally employs needles to inject donor cells into tissues to treat certain diseases. However, it is difficult for the current method to achieve multiple parallel equidistant injections, which are ideal for cell therapy. This paper presents a new cell transplantation method using an array of ultrathin microneedles. The main characteristic of the needles is their high aspect ratio: each needle is 500 µm long, and has a 50 µm diameter and a very thin wall (2 µm-thick SiO2 and 1.5 µm-thick Si3N4). An array of such microneedles was successfully used to inject fluorescently labeled Mardin---Darby canine kidney cells into rat liver tissue. Viability of the cells inserted using this method was verified after 5 days. Preliminary results show that this type of microneedle array can be used for cell therapy.
COBISS.SI-ID: 11884884
Exosomes, a type of nanovesicle, are distinct cellular entities specifically capable of carrying various cargos between cells. It has been hypothesized that exosomes, as an enriched source of biomolecules, may serve as biomarkers for various diseases. This review introduces general aspects of exosomes, presents the challenges in exosome research, discusses the potential of exosomes as biomarkers, and describes the contribution of microfluidic technology to enable their isolation and analysis for diagnostic and disease monitoring in LOC implementation.
COBISS.SI-ID: 12545876
A solid redox electrolyte based on organically modified silane matrix has been developed. The electrochromic device employing a solid electrolyte colors homogeneously, within less than 5 min when 240 nm cubic WO3 layer is employed. The results show that solidification of the electrolyte does not affect coloring kinetic, but the reduction of [TMFDS]2+ to TMTU at the Pt electrode significantly slows bleaching and reduces the self-bleaching of the device. Moreover, the EC device employing solid electrolyte is colorless in the bleached state (C, a and b values are below 10).
COBISS.SI-ID: 12428884
A 30?cm?×?30?cm double glazed window containing a PC multi-layer coating on a single glass pane has been developed. A single PC glass pane can be used to build a window with a low heat transfer coefficient, e.g. double or triple glazed unit, using existing technology. To develop the PC glass with a multi-layered coating that combines chromogenic and photoactive materials we employed sol-gel chemistry approaches and up-scaled the synthesis. The transmittance (Tvis) was reduced from 76% to 35% under outdoor solar radiation (750?W/m2) within 15?min.
COBISS.SI-ID: 12072020
Experimental study of in vivo transdermal insulin delivery by using hollow silicon microneedle array is presented. Case study was carried out on a healthy human subject in-vivo to determine the influence of delivery parameters on drug transfer efficiency. As microinjection device a hollow microneedle array (13x13 mm2) having 100 microneedles, (220 µm high) were designed and fabricated. Transfer efficiency was found to be predominantly limited by inability of viable epidermis to absorb and allow higher drug transport toward capillary rich region. Infusion of more concentrated insulin (200 IU/ml) exhibited similar blood glucose level drop (5-7%) compared to insulin of standard concentration (100 IU/ml), however, significant increase of serum insulin (40-50%) with respect to the preinfusion values.
COBISS.SI-ID: 33603801