Here, we reported the effect of drought on different physiological characteristics and the efficiency of isolation of chloroplasts from leaves of drought-stressed and control plants of common bean (Phaseolus vulgaris L.) in two cultivars differing in the response to drought. Negative effects of drought stress on the photosynthetic rate and chlorophyll fluorescence parameters of both cultivars were observed. The yield of isolated chloroplasts in control samples of both cultivars was higher compared to drought-stressed samples. The intactness of the isolated chloroplasts was checked and used for protein isolation. To estimate protein contaminants in the chloroplast proteins, western blot analysis was performed. Isolated chloroplast proteins were suitable for proteomic analysis by 2D-DIGE.
B.06 Other
COBISS.SI-ID: 5644392Here, we reported the proteomic approach with 2D-DIGE for analysis of drought-responsive proteins in chloroplast leaves of two cultivars differing in their response to drought, Tiber and more sensitive Starozagorski čern. Plants for proteome analysis were harvested on days 6 and 13 after the beginning of water withdrawal. Chloroplasts from control and drought-stressed plants leaves were isolated using Percoll gradient centrifugation. The yield of isolated chloroplasts in drought-stressed samples of both cultivars was lower compared to control samples in both cultivars. Proteins were labelled with G-Dyes developed for fluorescence 2D-DIGE technology. The DeCyder bioinformatic software was used for quantitative and statistical analysis of the 2D-DIGE images, where differences in protein abundance between control and stressed plants and between the cultivars were determined.
B.06 Other
COBISS.SI-ID: 9064313