Microscopy methods with resolution better than a micrometre were implemented in the scope of the project. This method was used to study the deviation of a reference analytical standard. We have unambiguously shown that the unexpected turbidity of the reference samples is caused by micron-sized air bubbles and not protein particles, as first suspected. With this solution we significantly reduced the cost and duration of the study.
F.14 Improvements to existing production methods and tools or processes
M. Zidar defended his PhD in 2020 at UL FMF under the mentorship of M. Ravnik with the work Analysis and prediction of aggregation and degradation in protein-based biopharmaceuticals, whereby an important part of the doctoral work was performed within this ARRS project, especially the experimental determination of the bulk and surface affected aggregation and modelling of the aggregation kinetics. In 2020, under the mentorship of M. Ravnik, N. Kralj received her master's degree from UL FMF with the work Modeling time and temperature dependence of differently charged protein variants, and U. Markoja received his master's degree in 2018 with the work Modeling protein aggregation as effective colloidal particles, which are both topics related to the research of the reported project.
D.09 Tutoring for postgraduate students
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