The present study evaluated the effects of lactational exposure to nondioxin-like PCB-155 and dioxin-like PCB-169, individually and in combination, on pubertal rat femur development and its biomechanics. After offspring delivery, Wistar rat mothers were divided into four groups, i.e., PCB-169, PCB-155, PCB-155+169 and control, and were administered PCBs intraperitoneally. Data on bone geometry, biomechanics and mineral composition were obtained by analysis of femurs from 42-day-old offspring by microCT scanning, three-point bending test and inductively coupled plasma mass spectrometry. Decreased somatic mass and femur size, i.e., mass, periosteal circumference and cross sectional area, were observed in the PCB-169 and PCB-155 groups. Additionally, lactational exposure to planar PCB-169 resulted in harder and more brittle bones containing higher amounts of minerals. Combined exposure to structurally and functionally different PCBs demonstrated only mild alterations in bone width and mineralization. To conclude, our results demonstrated that alterations, observed on postnatal day 42, were primarily induced by PCB-169, while toxicity from both of the individual congeners may have been reduced in the combined group.
COBISS.SI-ID: 4812666
The article describes a patient with a recently published disease molar-root incisor malformation (MRIM). In a patient with the autoimmune lymphoproliferative syndrome (ALPS), a dental clinical examination also confirmed MRIM abnormal morphology of the pulp cavities and roots of the tooth was recognized on all four of the first permanent molars (FPM). The teeth were extracted due to inflammatory complications and subjected to light microscopy, SEM evaluation and mineral density and elemental composition analyses. Histology of two PFMs revealed the presence of dentin-, bone- and cartilage-like tissues with abundant blood vessels occupying the majority of the pulp chamber. The root canals were obliterated with mineralized structures resembling pulp stones. Two different, highly mineralized abnormal tissues filling the majority of the pulp chamber revealed by SEM and confirming the diagnosis of MRIM displayed a mineral density and elemental composition similar to those of enamel and dentin, respectively. It appears likely that in addition to the complex medical history during early childhood in the present case, extensive lymphoid infiltrates that are possible in ALPS patients can be regarded as a cofactor in the development of MRIM by exerting considerable pressure on the developing tooth bud and providing cells capable of differentiating into diverse cell types.
COBISS.SI-ID: 4778874
Phototherapy is a treatment with different modalities of light, such as laser, lightemitting diode (LED), halogen lights or other light sources. Photobiomodulation is a type of phototherapy. Its primary effect is not thermal, as it is mainly due to the light being absorbed within chromophores of exposed cells resulting in increased cell metabolism and improved immunologic response of the tissue. The aim of our pilot study was to elucidate how phototherapy of salivary glands with polychromatic light or LED therapeutic light reduces caries risk factors in patients with high caries risk. Hypothesis: Polarized polychromatic noncoherent light or LED phototherapy reduces caries risk in high caries risk patients Methods: In prospective randomised study with placebo 40 patients with high caries risk according to Cariogram were assigned into four groups. In experimental groups the patients were irradiated with polarized polychromatic non-coherent light Bioptron, continuous LED light Ortholumm and same LED light Ortholumm in pulsed mode. For control group non-therapeutic low power visible light was used. In all patients light was administered extraorally bilaterally on the parotid and submandibular glands for 10 minutes and intraorally on the sublingual glands for 5 minutes, 3 times a week, for 4 weeks in a row. Every patient's caries risk was assessed according to Cariogram at the start and the end of therapy. Salivary caries risk factors were determined from saliva samples before the therapy, after two weeks, at the end of treatment and 4 weeks after the end of therapy. Results: At the end of the treatment, in group, irradiated with polarised polichromatic light (N = 9), and in group, irradiated with continuous LED light (N = 8), Streptococcus mutans and Lactobacillus CFU/ml counts were reduced and buffering capacity of saliva was increased (one-way repeated measures ANOVA, Dunnett’s test, p ( 0.05). At the end of the treatment, in group, irradiated with LED light in pulsed mode (N = 7), Streptococcus mutans CFU/ml counts were reduced, unstimulated salivary flow was increased and buffering capacity of saliva was increased (one-way repeated measures ANOVA, Dunnett’s test, p ( 0.05). In all three experimental groups caries risk at the end of phototherapy was lower (Wilcoxon test, p ( 0,05). In placebo control group (N = 12) there were no statistically significant differences between parameters during and after the therapy. Conclusions: Phototherapy can reduce the amount of cariogenic bacteria in saliva and improve some salivary parameters thus diminishing the risk for new caries lesion formation. The results from our study confirm the hypothesis that phototherapy with polarized polychromatic non-cocherent light or LED light reduces caries risk factors in patients with high caries risk.
COBISS.SI-ID: 3302523
The purpose of this study was to determine whether oral mucosa wounds in rats can be successfully treated with triamcinolone acetonide (TA), incorporated into liposomes. A round wound was inflicted on the oral mucosa of female Wistar rats divided into four groups of 12 animals. This wound was treated topically from day 1 with liposomes without the inclusion of TA and liposomes containing 0.01% or 0.05% TA. The wounds of the animals in the control group were not treated. Polymethyl metacrylate was used as an ointment for mixing in liposomes. The size of the wound was measured until day 6. The area of inflammatory infiltrate under the wound was evaluated by histopathology, the expression of iNOS (inducible nitric oxide synthase) enzyme under the wound was evaluated by immunohistochemistry until day 6. On the sixth day of experiment, the size of the wound and the area of the inflammatory infiltrate was the smallest in the group receiving empty liposomes (EL). Expression of iNOS was the most reduced in the group receiving EL. We conclude that oral mucosa wounds can be successfully treated with liposomes, although the incorporated drug triamcinolone would not be the appropriate drug for treating wounds of traumatic origin.
COBISS.SI-ID: 4839034
Collagen matrices (CMs) could be used instead of connective tissue grafts (CTGs) for treatment of gingival recession (GR). The authors aimed to compare clinical outcomes after treatment of isolated GR affecting both maxillary canines of 10 patients (n = 10) with CM (OsteoBiol Derma) or CTG in conjunction with coronally advanced flap. After 12 months, CM and CTG showed complete correction in 7/10 and 10/10 of sites, respectfully, and percentage of root coverage was 85% ± 24% and 100%, respectively. For treatment of isolated GR, CM is a viable alternative to CTG.
COBISS.SI-ID: 5883052