Objectives: To assess arch width, palatal surface area, and volume in surgically treated unilateral cleft lip and palate (UCLP) in mixed dentition children in comparison with non-cleft lip and palate (NCLP) children using a 3D laser scanning. Materials and Methods: 38 subjects (Caucasian origin), 5.63-11.9 years of age (mean, 9.33 ± 1.67 years), were included. 19 in each group (UCLP and NCLP). Digital dental casts were obtained using a 3 Shape R700 laser scanner. Intercanine and intermolar widths (cusp and gingival levels), palatal surface area and volume were measured. An independent sample Student's t-test and an ANOVA were undertaken with significance level set as P ( 0.05. Results: Intercanine widths at the cusp (5.60 mm; P ( 0.001) and at the gingival level (3.11 mm; P = 0.014), palatal area (141.5 mm2; P = 0.009) and volume (890.7 mm3; P = 0.029) were significantly lower in the UCLP compared to the control group. Limitations: A smaller part of the subjects was in late mixed dentition phase. To overcome this limitation a matched control group was used. In seven subjects with UCLP, some teeth were missing, which might have had an influence on the dental measurements. However, these subjects could not be excluded because eliminating more severely affected subjects, would have introduced bias. Conclusions: Three-dimensional evaluation of the maxillary arch and palate highlighted significant differences between UCLP and non-UCLP subjects in mixed dentition phase, suggesting that orthopaedic maxillary expansion is advisable in UCLP.
F.21 Development of new health/diagnostic methods/procedures
COBISS.SI-ID: 33205465Background/objectives: Facial soft tissues changes during growth roughly tend to mimic the underlying hard tissues, but not completely. The aim of this mixed longitudinal study was to assess facial growth among pre-pubertal and pubertal subjects without malocclusion using a noninvasive three-dimensional laser scanning system. Subjects/methods: Fifty-nine subjects (30 females and 29 males) aged at baseline 5.4-8.9 years with normal occlusion were clustered into the younger, older pre-pubertal, and pubertal groups according to age and the absence/presence of a standing height growth spurt. Three-dimensional facial images were obtained using laser scanners for five consecutive years. Several transversal, sagittal, and vertical parameters were assessed for between and within group comparisons. Results: Significant overall changes of almost all parameters were seen within each group (P ( 0.05) without any group differences (P ) 0.05). The younger pre-pubertal group showed greater annual growth rates of lip prominence; both pre-pubertal groups showed greater rates in facial middle third height. The pubertal group showed greater annual rates in facial profile angle changes during the growth peak. Limitations: A high standing height increment (7 cm) was used as the threshold for subject allocation in the pubertal group. Conclusions: Soft tissue facial growth has generally similar amounts and rates irrespective of the pubertal growth spurt. Pre-pubertal subjects show greater annual rates of facial middle third height changes while pubertal subjects show greater annual rates of chin protrusion.
F.22 Improvement to existing health/diagnostic methods/procedures
COBISS.SI-ID: 32498649Sensorineural hearing loss (SNHL) is a heterogeneous family of hearing disabilities with congenital (including genetic) as well as acquired etiology. Congenital SNHL of genetic etiology is further sub-divided into autosomal dominant, autosomal recessive and X-linked SNHL. More than 60 genes are involved in the etiology of autosomal recessive non-syndromic hearing loss (ARNSHL) commonly manifesting as heterogeneous pre-lingual profound to severe non-progressive clinical phenotype. ILDR1-dependent ARNSHL (DFNB42, OMIM: # 609646) is a very rare sub-type of hearing disability, with unknown prevalence, caused by function-damaging genetic variants in ILDR1 gene reported in families of Middle-Eastern origin. ILDR1 (Immunoglobulin-Like Domain-containing Receptor 1) is involved in the development of semicircular canal, tricellular tight junction and auditory hair cells. An apparently non-consanguineous family of European ancestry with two affected siblings with profound progressive hearing loss characterized in their infancy and successfully treated with cochlear implants (CI) is presented. Genetic analysis of common ARNSHL genetic causes in the population of origin was negative, thus the next-generation sequencing (NGS) and family segregation analysis to identify underlying causative genetic variant was performed. Unexpectedly and atypical for the population of origin a homozygous non-sense variant ILDR1 c.942C ) A (p.Cys314Ter) inherited from both heterozygous parents was identified in both patients. Contrary to the commonly reported phenotype, indices of a progressive hearing loss and potential compensatory mechanism of vestibular function were revealed with the analysis of clinical data. The utilization of NGS was demonstrated as an invaluable tool for the detection of atypical rare variants in diagnostics of unidentified hearing loss disorders.
F.17 Transfer of existing technologies, know-how, methods and procedures into practice
COBISS.SI-ID: 3926188The study has been introduced in 5th European Congress on Microbial Biofilms. Conventional treatment of periodontal disease does not solve the high incidence of re-colonization of periodontal pockets by periodontal pathogens and a consequent quick reemergence of the disease. Here, we introduce an innovative concept of indigenous probiotic incorporation into nanofibers in the form of spores for local treatment of periodontal disease. The isolated bacterial strain 25.2.M, belonging to genus Bacillus, was isolated from oral microbiota of healthy volunteers and was confirmed to be a potential probiotic due to its antimicrobial and good colonizing properties.
F.02 Acquisition of new scientific knowledge
COBISS.SI-ID: 30789671Mandibular prognatism and maxillary retrognatism resulting in skeletal Class III (SCIII) require the combination of orthodontic and surgical treatment. Many studies of the facial soft tissues with threedimensional analysis, ultrasound diagnostics, and speech analysis were done in our department. The aim of one of this prospective study was to verify objectively whether post-operative changes occur in regions not directly affected with surgical movements of the underlying jaw bones. According to the type of surgery - BSSO set-back of the mandible, Le Fort I advancement of maxilla or a combination of both, they were classified into three groups. The pre- and post-operative optical scans were registered with regional best-fit on the area of forehead and both orbits. According to the expectations, changes were the greatest in the regions where the underlying bones were moved, but changes were found over the whole face; confirm the concept of the facial soft tissue mask acting as one unit. We also determined ratios of soft tissues changes after sagittal skeletal bone changes for whole face and prooved the significantly bigger tongue volumes in SCIII patients. This morphological and physiological knowledge can improve the overall treatment of SCIII orthognatic deformities.
B.04 Guest lecture