The first Slovenian manual and textbook for doctors. The author is a renowned Slovenian hematologist, prof.dr.Samo ZVER, (author, editor). Nujna stanja v hematologiji : priročnik za zdravnike. Ljubljana: Pliva, 2019. ISBN 978-961-91586-4-7. [COBISS.SI-ID 301728768]
D.10 Educational activities
COBISS.SI-ID: 301728768The textbook Biotechnology of Mesenchymal Stem Cells describes in a transparent and informative way the properties of mesenchymal stromal / stem cells (MSCs), the procedures for their in vitro amplification required for the production of cellular drugs for advanced treatment, new insights into their secretions and current areas of their clinical use. .
D.10 Educational activities
COBISS.SI-ID: 303257856The aging of multicellular organisms is a complex process, which is a result of various mutually complementary causes. One of these causes is the aging of stem cells. The biological function of stem cells is the replacement of cells that are lost due to illness, injury or normal fluctuations in the maintenance of tissue homeostasis. Molecular mechanisms involved in stem cell aging are similar to those involved in the aging of somatic cells. They include DNA damage and mutations, cell senescence, stem cell exhaustion, telomere shortening, epigenetic changes (alterations of histones and DNA and the consequent dysregulation of gene expression), changes in microRNAs, changes in metabolism, nutrient sensing, decline in mitochondrial integrity and biogenesis, alterations in microenvironment, accumulation of paracrine factors, and loss of cell polarity and proteostasis. Stem cells have developed special mechanisms that compensate for age-related accumulations of errors and they manage to maintain their stemness for a long time, however, they are able to keep cells in a good condition only for a limited period. This article describes the various mechanisms of stem cell aging and their consequences.
F.03 Increased qualifications of the research and development staff
COBISS.SI-ID: 34284761Previously, we have shown platelet lysate (PL) can be used as a nonxenogeneic serum supplement for generation of monocyte-derived dendritic cells (DCs). Since DC-based activation protocols are extremely sensitive to microenvironmental changes such as replacement of culture medium, we wanted to examine the behavior of DCs cultured in the presence of PL under various type-1 activation conditions and assess their type 1 polarization capacity. We compared the quality of DCs cultured in 10% PL-supplemented RPMI medium (plDCs) with clinical-grade DCs obtained using commercially available serum-free medium (sfDCs), frequently used in established DC vaccine protocols. The DC maturation protocols consisted of either monophosphoryl lipid A/IFN-[gamma], poly I:C/TNF-[alpha]/IFN-[alpha] or poly I:C/R848. In general, plDCs were inferior to sfDCs in most aspects of their functional type 1 polarization characteristics. After maturation, the expression of co-stimulatory, HLA class II and lymph node-homing molecules was strongly up-regulated, with some noticeable differences. The expression of CD80 and CD86 was more extensive on plDCs, which was particularly evident in case of CCR7. However, after observing their functional capacity, plDCs had significantly lower allo-stimulatory capacity both in terms of CD4+ and CD8+ T cell stimulation. The high expression of CCR7 corresponded to higher CCL-19 directed DC migration of plDCs compared to sfDCs. Finally, their capacity to induce granzyme B...
F.02 Acquisition of new scientific knowledge
COBISS.SI-ID: 34355161