The previously developed GoMapMan web application (COBISS.SI-ID 2966607) has been upgraded to include the cacao plant (in addition to previously existing Arabidopsis, potato, tomato, rice and tobacco). The connections within the application ease interpretation of high-throughput data sets and knowledge transfer from model species to crops.
COBISS.SI-ID: 3869775
Published paper presents the set-up of virus-induced gene silencing (VIGS), based on Tobacco rattle virus (TRV) in wild potato relatives for functional studies in potato–virus interactions. Screening of a large set of wild potato relatives (different Solanum species and their clones) for their susceptibility to VIGS was performed by silencing PDS gene. We identified several responsive species and further tested susceptibility of these genotypes to potato virus Y (PVY) strain NTN and N. In some species we observed that the presence of empty TRV vector restricted the movement of PVY. Fluorescently tagged PVYN-GFP spread systemically in only five of tested wild potato relatives. Based on the results, Solanum venturii (VNT366-2) was selected as the most suitable system for functional analysis of genes involved in potato–PVY interaction. The system was tested by silencing two different plant immune signalling-related kinases, StWIPK and StMKK6. Silencing of StMKK6 enabled faster spreading of the virus throughout the plant, while silencing of WIPK had no effect on spreading of the virus. The system employing S. venturii (VNT366-2) and PVYN-GFP was shown to be a suitable method for fast and simple functional analysis of genes involved in potato–PVY interactions.
COBISS.SI-ID: 3877967