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The use of methoxyamine to study abasic site induced mutagenesis in bacterial strains

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Research activity

Code Science Field Subfield
4.06.04  Biotechnical sciences  Biotechnology  Microbe biotechnology 

Code Science Field
B220  Biomedical sciences  Genetics, cytogenetics 
Keywords
methoxyamine, antimutagen, DNA repair, apurinic/apirimidinic sites, alkylating agents, E. coli
Evaluation (rules)
source: COBISS
Evaluation of bibliographic research performance indicators according to ARIS methodology
Citations Citations for bibliographic records in COBIB.SI that are linked to records in citation databases
source: WoS source: Scopus source: COBISS
Researchers (1)
no. Code Name and surname Research area Role Period No. of publications
1.  09892  PhD Metka Filipič  Biology  Head  1998 - 1999  0 
Organisations (1)
no. Code Research organisation City Registration number No. of publications
1.  0105  National Institute of Biology  Ljubljana  5055784  0 
Abstract
Abasic sites are common intermediates involved in processes of mutagenesis and carcinogenesis. They are formed spontaneously and their formation is enhanced by various chemical and physical agents. Their role in these processes is not jet totally understood. Methoxyamine (MX) is a nucleofilic reagent able to specifically react with abasic sites. It was shown that MX is able to protect mammalian cells against cytotoxicity, mutagenicity and sister chromatide exchanges induced by SN1 alkylating agents. These results suggested that abasic sites are important component of alkylation induced mutagenesis. We propose the use of different E. coli strains which are impaired in different repair functions to investigate the formation and processing of AP sites in vivo. Bacterial strains offer the advantage, as compared to mammalian cells, because of availability of a wide spectrum of mutant strains impaired in different genes coding for repair enzymes. The use of these strains will allow to elucidate the role of different DNA repair functions in the formation and processing of abasic sites. Methoxyamine will be used as a tool to identify AP site mediated mutagenesis and its use as a molecular probe in vivo for AP site formation will be investigated.
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