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Equipment source: ARIS

Leica System for non-linear nanoscopy in tandem configuration

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Purpose of equipment
An upright nonlinear microscope is used to monitor and quantification of physiological processes in intact tissues and organs. The basis of this microscope enables mounting of biological samples of bigger dimensions and at the same time utilizes deep-penetrating infrared laser light to excite fluorescence. Handling of the laser beam used for fluorescence excitation can be relatively slow to improve the high spatially resolved morphological images or monitoring of relatively slow physiological processes. On the other hand we can move the laser beam over the sample using a high speed mode, which enables monitoring of the physiological processes with millisecond time resolution. The detector side consists of a classical system of high gain photodiodes with a possibility of spectral resolution. The major advance in detection of the faintest signals of fluorescence or bioluminescence comes from the direct mounting the system of non-descanned high resolution photodiodes.
Access of equipment
Responsible for equipment: PhD Marjan Slak Rupnik
URL: www.cipkebip.org
Prof.Dr. Marjan Slak Rupnik, University of Mariboru, Medical faculty, Ljubljanska 5, 2000 Maribor
Research projects (1) Legend
no. Code Title Period Head No. of publications
1.  N3-0170  Significance of exocrine-endocrine pancreatic interactions in health and disease  7/1/2021 - 6/30/2024  PhD Jurij Dolenšek  0 
ARIS research and infrastructure programmes (2) Legend
no. Code Title Period Head No. of publications
1.  P3-0396  Cell and tissue networks  1/1/2015 - 12/31/2018  PhD Marjan Slak Rupnik  0 
2.  I0-0048  Analiza in proizvodnja proteinov in biološko aktivnih molekul medicinskega pomena (Slovene)  1/1/2013 - 12/31/2014  PhD Dušan Turk  0 
Organisations (1)
no. Code Research organisation City Registration number No. of publications
1.  2990  Center of excellence for integrated approaches in chemistry and biology of proteins, Ljubljana  Ljubljana  3663388  0 
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